Strain engineering

In Recombination we trust

Explogen combines the extensive expertise in genetics, genomics, and metabolism of microorganisms with the in-house developed genetic tools for efficient and precise strain engineering. Our technologies are based on homologous and site-specific DNA recombination processes combined with cost-efficient end-sequenced cosmid libraries construction. The in-house developed cosmid vectors built around a versatile reporter system allows direct selection of secondary cross-over clones facilitating the mutant identification. This together makes the process of strain engineering fast and efficient, independently whether large deletion or single point mutation should be introduced.

Showcase 1:

Natural products biosynthesis “clean” host strain just in one year:
15 regions ranging from 9 to 29 kb were deleted from the chromosome of model bacteria species removing all major natural products biosynthetic gene clusters, significantly simplifying secondary metabolites profile of the strain.

Strain Engineering Services:

Custom strain engineering project based on Customer’s strain and its final desired properties. This includes:

• - Adapting the Explogen’s technology for the target strain.
• - Cosmid library construction, end-sequencing and genome mapping.
• - Construction of cosmid clones for gene or genomic region deletion, SNP introduction, promoter engineering, etc.
• - Introduction of desired mutations into the strain.
• - Verification by genome sequencing (on request).

Standard strain engineering project.

Explogen offers a set of ready-to-use cosmid library clones for engineering of model strains and antibiotics producers (click on strain name to see the cosmid library)*:

• - Pseudomonas putida table
• - Rhodobacter sphaeroides
• - Bacillus (Priestia) megaterium
• - Bacillus thuringiensis
• - Corynebacterium glutamicum
• - Pseudomonas fluorescens
• - Pseudomonas syringae
• - Comamonas testosteroni
• - Staphylococcus aureus
• - Streptomyces albidoflavus (albus)
• - Streptomyces lividans
• - Streptomyces kanamyceticus (kanamycin) table
• - Streptomyces cinnamonensis (monensin) table
• - Saccharopolyspora erythraea (erythromycin) table
• - Actinoplanes teichomyceticus (teicoplanin) table

Order Options:

- Unmodified clone of interest
- Custom modification of the clone of interest with gene knock-out, knock-in, introduction of SNP, promoter, RBS, other regulatory elements.
- Complete engineering project of different complexity: from design to the final strain verification.

* JBrowse 2: a modular genome browser with views of synteny and structural variation. Genome Biology (2023). https://doi.org/10.1186/s13059-023-02914-z